ABSTRACT
Aim To explore the effects of Zhimu-Huangbai herb pair( ZB) on insulin resistance. Methods The rat model of experimental T2DM was induced by intraperitoneal injection of streptozotocin after feeding with high fat and sucrose forage. Rats with T2DM were randomly divided into normal group, model group, metformin group and high-, low-dose ZB group(4. 5, 1. 5 g • kg"1) , with corresponding drugs administered orally by gastric gavage once a day for four weeks. The body weight, fasting blood glucose ( FBG ) , serum insulin level ( FINS ) and triglyceride content(TG) were measured, and the insulin sensitivity index(ISI) and insulin resistance index ( Homa-IR) were calculated. The HepG2 cells were treated with palmitate and oleate to induce insulin resistance, then divided into six groups: Control group, model group, metformin group and high-, middle-, low-dose ZB group(384, 192, 96 mg • L'1). Glucose consumption and TG content were assayed 24 h after administration. Results ZB not only markedly lowered the level of FBG, FINS, TG content (P <0. 05) and insulin resistance index ( P < 0. 01 ) , increased insulin sensitivity index(P <0. 05) in rats with T2DM, but also significantly decreased TG content (P <0. 05) and increased glucose consumption ( P < 0. 05 ) in HepG2 cell model with insulin resistance. Conclusions ZB can promote glucolipid metabolism and improve the insulin resistance by increasing insulin sensitivity.
ABSTRACT
Objective To explore the regional parameters of triple screening model of Down syndrome in the second trimester in Jinhua City.Methods A total of 20 232 second trimester pregnant women with single fetus (gestational age at 15 -20 +6 weeks)was enrolled,and their serum samples were determined by American Perkin Elmer company Auto DELFIA automatic time -resolved fluorescence immunoassay analyzer for Down syndrome screening with triple markers, namely AFP,free β-hCG and uE3 .The risks of Down syndrome were evaluated by Lifecycle 3.2 software.And the risks of Down syndrome were re -calculated by local statistical median equations.Pregnant women were suggested to receive amniotic fluid fetal karyotype analysis if the risk of Down syndrome were equal or above 1 /270.Results Local median marker levels were significantly higher than the software built -in median levels (P <0.01).Both true -positive detection rates (sensitivity)were 87.50%.The false positive rate of local median equations was 4.24%,while the built -in median equations was 4.74%.Conclusion There are significant differences on the race and region by using the LifeCycle 3.2 median equations.The local equations may lower the false positive rate.